Advances In Biochemical Engineering Biotechnology - download pdf or read online

By Bo Mattiasson, Lei Ye

Managed radical polymerization options for molecular imprinting, via Mark E. Byrne
From bulk polymers to nanoparticles, via Lei Ye
Post-imprinting and in-cavity functionalization, by means of Toshifumi Takeuchi
Characterization of MIPs (affinity, selectivity, web site heterogeneity…), via Richard Ansell
Theoretical features and machine modelling, via Ian Nicholls
MIPs in aqueous environments, through Bin Lu
MIPs for binding macromolecules, via Kenneth J. Shea
Solid section extraction, by means of Ecevit Yilmaz
Sensors, by way of Sergey A. Piletsky
MIPs for catalysis and synthesis, through Marina Resmini
Wastewater therapy, via Bo Mattiasson
MIPs as instruments for bioassays, biotransformation and drug supply, by way of Meiping Zhao

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The early work in the mid-1960s screened this Hnl against more than 60 aldehydes and developed a comprehensive overview of its substrate acceptance [54–56]. Latterly, numerous groups, including those of van der Gen, Effenberger Biocatalytic Applications of Hydroxynitrile Lyases 41 and Kanerva have applied this enzyme in the synthesis of chiral cyanohydrins, although for their extensive applications of the PaHnl the group of van der Gen is the most cited. They have applied benzaldehyde (3) to this enzyme and obtained good conversion (95% in 40 min at 0 °C), and the corresponding cyanohydrin was formed with excellent selectivity [57] (Table 2).

4 Immobilisation Techniques and On-Line Production of Cyanohydrins . . . . . . . . . . . . . . . 48 4 Enantiopure Cyanohydrins as Intermediates for the Synthesis of Bioactive Compounds . . . . . . . . . . . . 2 Chemical Transformations . . . . . . . . . . . . 49 Unsaturated Cyanohydrins . . . . . . . . . . . . 51 5 Conclusions and Outlook 6 References . . . . . . . . . . . . . . . . 53 . . . . . . . . . . . .

This mechanism is currently an issue of debate and more recently a modified mechanism has been discussed [52]. 1 (R)-Cyanohydrins The two established Hnls, those from L. usitatissimum and P. amygdalus, have found biocatalytic applications for the production of (R)-cyanohydrins. The former of these Hnls is the least widely applied, the natural substrates being acetone cyanohydrin or (R)-2-butanone cyanohydrin (Table 1) [28]. Although an improved procedure for the purification of this enzyme has been reported [27] it is still only available in limited quantities (from 100 g of seedlings approximately 350 U of enzyme are obtained).

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