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Extra resources for Analytical Biochemistry
These authors found only minor differences in the K /A ratios of immature and adult B cells of either CBNN or normal mice, with A-bearing cells representing only a minor fraction of the B cells. Although the findings in the studies of the F, mice of Golding et al. (1980) are most provocative, the extensive documentation of the reactivity of the anti-A reagent used in the latter two studies rules out any significant differences i n the K / A ratios of CBA/N and normal mice. Further studies to identify the additional specificities contained in these serum which distinguished immune-defective F, mice from normals would be most interesting.
A comparison of the helper activity of graded numbers of activated T cells froin abnormal male (CBA/N x DBA/2)F, ( 0 )or normal male (DBA/2 x CBA/N)F, (0) mice. , 1979). adoptive transfer experiments (Bottomly and Mosier, 1979). I n these studies lethally irradiated (CBA/N x BALB/c)F, mice were used as recipients of B and/or T cells derived from immune-defective F, male or normal female mice. F, male T cells were able to support a PC response by F, female B cells which was quantitatively similar to that of F, female T cells.
34 IHWIN SCHER 160 - 140 - 120 - 1w - F1Q LPS / Antip / I'/' I 2 t 60 101 105 106 107 SPLEEN CELLS PER CULTURE FIG. 8. , 1978b). D. SUSCEPTIBILITY TO TOLERANCE The susceptibility of CBA/N B cells to tolerance induction was of particular importance in view of the functional defects demonstrated in these cells when analyzed with TI-2 antigens or B-cell mitogens. A number of approaches have been employed in studies on this subject (E. , 1978a,b). In early studies it had been demonstrated that a larger proportion of neonatal splenic B cells and a smaller proportion of adult bone marrow B cells were sensitive to tolerance induction when exposed to a tolerogen i n vitro (E.