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Extra resources for Covalent analogues of DNA base-pairs and triplets. Part 2[c] Synthesis and cytostatic activity of bis(purin-6-yl)acetylenes, -diacetylenes and related compounds

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We are currently screening M2 mutagenized seed of the Arabidopsis ecotype CoI-O by inoculating with DC3000 (avrRpt2). The seed has been mutagenized with the mutagen diepoxybutane (DEB), which has been shown to cause deletions in other organisms. This is especially significant as it is now possible to clone genes from Arabidopsis thaliana using the protocol of genomic subtraction (19). Along these lines we have also developed a rapid and efficient screen to identify mutations. Basically, this procedure involves dipping whole plants into a bacterial suspension of 5xl08 cfu/ml of Pseudomonas sryingae pv.

S. pv. tomato library that yielded a weak hypersensitive reaction on 9 of the 10 standard soybean differential cultivars, but it proved difficult to subclone the phenotype to a small DNA fragment. s. pv. tomato hrp gene cluster. , 1988). Our recent results, obtained collaboratively with C. Boucher and B. s. pv. tomato clone is indeed conferred by a ca. s. pv. , 1990). Kobayashi et al. s. pv. tomato and showed that it was the first of five tandem open reading frames which appear to be organized as an operon.

S. pv. , 1990 and unpublished). P. syringae pv. s. pv. glycinea only makes exceedingly low levels and E. coli does not produce detectable activity in the absence of the cloned avrD gene. The elicitor activity has proved to be somewhat unstable during purification, but crystalline preparations have recently been obtained that elicit the HR only in leaves of soybean cultivars carrying Rpg4 (minimal concentration, ca. 10 ng in 10 ~l water per injection site for production of a visible HR on Rpg4 primary soybean leaves).

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