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By Louise O'Connor

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W. (2000) Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Appl. Environ. Microbiol. 66, 4641–4648. 13. Jakobsen, K. , et al. (2002) Discovery of the toxic dinoflagellate Pfiesteria in northern European waters. Proc. Royal Soc. Lond. B. 269, 211–214. 14. , and Radstrom, P. (1997) Removal of PCR inhibitors from human fecal samples trough the use of an aqueous two-phase sample preparation prior to PCR. J. Microbiol. Methods 28, 159–167. 15.

Bruin, G. J. (2000) Recent developments in electrokinetically driven analysis on microfabricated devices. Electrophoresis 21, 3931–3951. DNA Purification for Diagnostics 35 50. Rochelle, P. (2001) Environmental Molecular Microbiology: Protocols and Applications. Horizon Scientific Press, Wymondham, UK, p. 264. 51. Watson, R. J. and Blackwell, B. (2000) Purification and characterization of a common soil component which inhibits the polymerase chain reaction. Can. J. Microbiol. 46, 633–642. 52. , and Megraud, F.

Spotting of Glass Slides 1. Spot the slides using an Affymetrix 417 Arrayer equipped with 125 µm pins, which results in spots approx 200 µm diameter. 2. Before and during spotting, ambient temperature and air humidity must be controlled and maintained at 20ЊC and 50 to 60% relative humidity, respectively (see Note 7). 3. The spotting layout (see Note 8) is determined by the print head geometry and the well positions of specific DNA oligos within the plates. Each slide can easily hold two arrays, which require a 22 ϫ 22-mm area for on-chip PCR.

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